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  • 221. Genome-wide analysis of cytochrome P450 genes in Citrus clementina and characterization of a CYP gene encoding flavonoid 3 '-hydroxylase

    文献信息:Liu, Xiaojuan, Gong, Qin, Zhao, Chenning, Wang, Dengliang, Ye, Xianming, Zheng, Guixia, Wang, Yue, Cao, Jinping and Sun, Chongde等.  请登录

    简介:Cytochrome P450s (CYPs) are the largest family of enzymes in plant and play multifarious roles in development and defense but the available information about the CYP superfamily in citrus is very limited. Here we provide a comprehensive genome-wide analysis of the CYP superfamily in Citrus clementina genome, identifying 301 CYP genes grouped into ten clans and 49 families. The characteristics of b...

    222. Laser capture microdissection-based spatiotemporal transcriptomes uncover regulatory networks during seed abortion in seedless Ponkan (Citrus reticulata)

    文献信息: Lu, Liqing, Yang, Haijian, Xu, Yanhui, Zhang, Li, Wu, Juxun and Yi, Hualin等.  请登录

    简介:Seed abortion is an important process in the formation of seedless characteristics in citrus fruits. However, the molecular regulatory mechanism underlying citrus seed abortion is poorly understood. Laser capture microdissection-based RNA-seq combined with Pacbio-seq was used to profile seed development in the Ponkan cultivars 'Huagan No.4' (seedless Ponkan) (Citrus reticulata) and 'E'gan No.1' (s...

    223. MicroRNA miR171b Positively Regulates Resistance to Huanglongbing of Citrus

    文献信息:Lv, Yuanda, Zhong, Yun, Jiang, Bo, Yan, Huaxue, Ren, Shuang and Cheng, Chunzhen等.  请登录

    简介:Huanglongbing (HLB) is one of the most severe citrus diseases in the world, causing huge economic losses. However, efficient methods of protecting citrus from HLB have not yet been developed. microRNA (miRNA)-mediated regulation of gene expression is a useful tool to control plant diseases, but the miRNAs involved in regulating resistance to HLB have not yet been identified. In this study, we foun...

    224. Citrus improvement for enhanced mineral nutrients in fruit juice through interspecific hybridization

    文献信息:Singh, Narendra, Sharma, R. M., Dubey, A. K., Awasthi, O. P., Saha, Supradip, Bharadwaj, C., Sharma, V. K., Sevanthi, Amitha Mithra, Kumar, Amrender a等.  请登录

    简介:The present study aimed to determine the variation in mineral nutrition properties for fresh juice of 16 new citrus (C. maxima [Burm. f.] Osbeck x Citrus sinensis [L.] Osbeck) and parental genotypes. The correlation and in-heritance estimate for fruit juice mineral nutrients was carried out. The results of present study showed that citrus fruits are valuable source of supplementing essential miner...

    225. PRpnp, a novel dual activity PNP family protein improves plant vigour and confers multiple stress tolerance in Citrus aurantifolia

    文献信息:Singh, Sweta, Chaudhary, Chanderkant, Bharsakale, Rushikesh D. D., Gazal, Snehi, Verma, Lokesh, Tarannum, Zeba, Behere, Ganesh T. T., Giri, Jitender, 等.  请登录

    简介: Under field conditions, plants are often simultaneously exposed to several abiotic and biotic stresses resulting in significant reductions in growth and yield; thus, developing a multi-stress tolerant variety is imperative. Previously, we reported the neofunctionalization of a novel PNP family protein, Putranjiva roxburghii purine nucleoside phosphorylase (PRpnp) to trypsin inhibitor to cater to ...

    226. Overexpression of the salicylic acid binding protein 2 (SABP2) from tobacco enhances tolerance against Huanglongbing in transgenic citrus

    文献信息:Soares, J. M., Weber, K. C., Qiu, W., Mahmoud, L. M., Grosser, J. W. and Dutt, M.等.  请登录

    简介:Overexpression of the salicylic acid binding protein 2 (SABP2) gene from Tobacco results in enhanced tolerance to Huanglongbing (HLB; citrus greening disease) in transgenic sweet oranges. Huanglongbing (HLB), the most destructive citrus disease, is caused by Candidatus Liberibacter asiaticus (CaLas). Currently, no cure for this disease exists, and all commercially planted cultivars are highly susc...

    227. Molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in Citrus sinensis

    文献信息: Song, Shipeng, Liu, Hui, Miao, Luke, He, Li, Xie, Wenzhao, Lan, Hong, Yu, Changxiu, Yan, Wenkai, Wu, Yufeng, Wen, Xiao-Peng, Xu, Qiang, Deng, Xiuxin 等.  请登录

    简介:Citrus sinensis is the most cultivated and economically valuable Citrus species in the world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to the small size of chromosomes, and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome. Here, we employ fluorescence in situ hybri...

    228. Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation

    文献信息:Su, H., Wang, Y., Xu, J., Omar, A. A., Grosser, J. W., Calovic, M., Zhang, L., Feng, Y., Vakulskas, C. A. and Wang, N.等.  请登录

    简介:Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is a destructive citrus disease worldwide. Generating disease-resistant cultivars is the most effective, environmentally friendly and economic approach for disease control. However, citrus traditional breeding is lengthy and laborious. Here, we develop transgene-free canker-resistant Citrus sinensis lines in the T0 generation within 10 m...

    229. A self-regulated transcription factor CsWRKY33 enhances resistance of citrus fruit to Penicillium digitatum

    文献信息:Wang, Wenjun, Li, Ting, Chen, Jialin, Zhang, Xuan, Wei, Linli, Yao, Shixiang and Zeng, Kaifang等.  请登录

    简介:Green mold caused by Penicillium digitatum is a major postharvest disease in citrus fruit, but the molecular mechanism related to the induced disease resistance in citrus is still unclear. This study investigated the regu-latory function of CsWRKY33 on citrus disease resistance. The results showed that CsWRKY33 was located in the nucleus, and the transient overexpression of CsWRKY33 significantly ...

    230. Transcriptional regulatory mechanism of a variant transcription factor CsWRKY23 in citrus fruit resistance to Penicillium digitatum

    文献信息:Wang, Wenjun, Li, Ting, Chen, Qi, Yao, Shixiang and Zeng, Kaifang等.  请登录

    简介: As the most productive fruit in the world, there is a lack of research on disease resistance mechanisms in citrus. WRKY transcription factors are mediators of disease resistance in plants. In this research, CsWRKY23, which could respond to exogenous salicylic acid (SA) was identified. As a variant WRKY, the transient overexpression of CsWRKY23 in citrus peel enhanced the resistance to Penicillium...

    231. A citrus pulp mastication trait-related ABSCISIC ACID-INSENSITIVE 5-like protein promotes fruit softening via transcriptionally activating pectate lyase gene

    文献信息:Wang, Xun, Li, Yuan, Lu, Wen, Zhang, Mingfei, Deng, Honghong, Xiong, Bo, Liao, Ling, Tang, Yi, Lin, Lijin, Zhao, Junming and Wang, Zhihui Year: 2023等.  请登录

    简介:Mastication trait of citrus fruit is an important criterion to evaluate mandarin citrus fruit quality and decides its market value. However, the regulatory mechanism underlying the mastication trait formation is unclear. Here we isolated a bZIP family member, annotated to be "ABSCISIC ACID-INSENSITIVE 5-like protein 5", from fruits of two mandarin citrus cultivars with different mastication trait,...

    232. Genome-wide identification of the CsPAL gene family and functional analysis for strengthening green mold resistance in citrus fruit

    文献信息:Wei, Linli, Wang, Wenjun, Li, Ting, Chen, Ou, Yao, Shixiang, Deng, Lili and Zeng, Kaifang等.  请登录

    简介:Phenylalanine aminolyase (PAL) is a key rate-limiting enzyme. It catalyzes the initial step of the phenylpropane metabolic pathway. PAL is involved in the regulation of fruit diseases, but the specific function of the PAL gene in the regulation of postharvest fruit diseases is unknown. In this study, we identified four CsPAL members in citrus, including CsPAL1-CsPAL4. Based on physicochemical prop...

    233. The functional analysis of sugar transporter proteins in sugar accumulation and pollen tube growth in pummelo (Citrus grandis)

    文献信息:Xu, Weiwei, Liu, Ziyan, Zhao, Zeqi, Zhang, Shuhang, Li, Mengdi, Guo, Dayong, Liu, Ji-Hong and Li, Chunlong等.  请登录

    简介:Sugar transporter proteins (STPs) play vital roles in sugar transport and allocation of carbon sources in plants. However, the evolutionary dynamics of this important gene family and their functions are still largely unknown in citrus, which is the largest fruit crop in the world. In this study, fourteen non-redundant CgSTP family members were identified in pummelo (Citrus grandis). A comprehensiv...

    234. CsERF003, CsMYB7 and CsMYB102 promote cuticular wax accumulation by upregulating CsKCS2 at fruit ripening in Citrus sinensis

    文献信息:Yang, Hongbin, Zhang, Mingfei, Li, Xin, Zhu, Zhifeng, Xu, Rangwei, Zhu, Feng, Xu, Juan, Deng, Xiuxin and Cheng, Yunjiang等.  请登录

    简介: Cuticular wax is a hydrophobic protective layer covering the surface of citrus fruit, which plays an important role in preventing fruit water loss and improving fruit appearance quality. Our previous study has demonstrated that CsKCS2 is highly expressed at fruit ripening and involved in fruit cuticular wax synthesis in Citrus sinensis. However, the upstream regulators of CsKCS2 and the transcrip...

    235. A bZIP transcription factor (CiFD) regulates drought- and low-temperature-induced flowering by alternative splicing in citrus

    文献信息:Ye, Li-Xia, Wu, Yan-Mei, Zhang, Jin-Xia, Zhang, Jin-Xin, Zhou, Huan, Zeng, Ren-Fang, Zheng, Wei-Xuan, Qiu, Mei-Qi, Zhou, Jing-Jing, Xie, Zong-Zhou, Hu等.  请登录

    简介:Drought and low temperature are two key environmental factors that induce adult citrus flowering. However, the underlying regulation mechanism is poorly understood. The bZIP transcription factor FD is a key component of the florigen activation complex (FAC) which is composed of FLOWERING LOCUS T (FT), FD, and 14-3-3 proteins. In this study, isolation and characterization of CiFD in citrus found th...

    236. Jasmonate activates a CsMPK6-CsMYC2 module that regulates the expression of beta-citraurin biosynthetic genes and fruit coloration in orange (Citrus sinensis)

    文献信息:Yue, Pengtao, Jiang, Zhenghua, Sun, Quan, Wei, Ranran, Yin, Yingzi, Xie, Zongzhou, Larkin, Robert M., Ye, Junli, Chai, Lijun and Deng, Xiuxin等.  请登录

    简介:Carotenoids are natural pigments that influence the color of citrus fruit. The red-colored carotenoid beta-citraurin is responsible for the peel color in "Newhall" orange (Citrus sinensis). Although jasmonates are known to regulate the biosynthesis and accumulation of carotenoids, their effects on beta-citraurin biosynthesis in citrus fruit remain unclear. Here, we determined that treatment with m...

    237. CcRR5 interacts with CcRR14 and CcSnRK2s to regulate the root development in citrus

    文献信息:Zhang, M. M., Wang, F. S., Hu, Z., Wang, X. L., Yi, Q., Feng, J. P., Zhao, X. C. and Zhu, S. P.等.  请登录

    简介: Response regulator (RR) is an important component of the cytokinin (CK) signal transduction system associated with root development and stress resistance in model plants. However, the function of RR gene and the molecular mechanism on regulating the root development in woody plants such as citrus remain unclear. Here, we demonstrate that CcRR5, a member of the type A RR, regulates the morphogenes...

    238. Citrus beta-carotene hydroxylase 2 (BCH2) participates in xanthophyll synthesis by catalyzing the hydroxylation of beta-carotene and compensates for BCH1 in citrus carotenoid metabolism

    文献信息:Zhang, Yingzi, Jin, Jiajing, Zhu, Shenchao, Sun, Quan, Zhang, Yin, Xie, Zongzhou, Ye, Junli and Deng, Xiuxin等.  请登录

    简介: As an essential horticultural crop, Citrus has carotenoid diversity, which affects its aesthetic and nutritional values. beta,beta-Xanthophylls are the primary carotenoids accumulated in citrus fruits, and non-heme di-iron carotene hydroxylase (BCH) enzymes are mainly responsible for beta,beta-xanthophyll synthesis. Previous studies have focused on the hydroxylation of BCH1, but the role of its p...

    239. Highly efficient hairy root genetic transformation and applications in citrus

    文献信息:Ma, H. J., Meng, X. Y., Xu, K., Li, M., Gmitter, F. G., Liu, N. G., Gai, Y. P., Huang, S. Y., Wang, M., Wang, N., Xu, H. R., Liu, J. H., Sun, X. P. an等.  请登录

    简介: Highly efficient genetic transformation technology is greatly beneficial for crop gene function analysis and precision breeding. However, the most commonly used genetic transformation technology for woody plants, mediated by Agrobacterium tumefaciens, is time-consuming and inefficient, which limits its utility for gene function analysis. In this study, a simple, universal, and highly efficient ge...

    240. Increasing the production of limonin 17-β-D-glucopyranoside in hairy roots of Citrus x paradisi. cv Duncan by overexpression of limonoid UDP-glucosyltransferase via Agrobacterium rhizogenes mediated

    文献信息:Cheraei, S., Eskandari, F., Yadollahi, A. and Zaare-Nahandi, F.等.  请登录

    简介:Limonin 17-beta-D-glucopyranoside (LG) is a terpenoid compound with a high medicinal value produced in different Citrus species. This substance has high anti-cancer and antioxidant properties and protects the liver from damage. Although LG is tasteless, many compounds related to it are very bitter, although these bitter substances also have significant medicinal properties. Usually, in most Citrus...

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