文献信息：曹鹏, 周金环, 王新亮, 李楚欣, 李佳欣, 姜培, 刘金香 and 宋震等.  请登录

简介：【目的】黄龙病（Huanglongbing,HLB）是一种毁灭性柑橘病害，主要由韧皮部杆菌属亚洲种（Candidatus Liberibacter asiaticus,CLas）侵染引起。本研究旨在探究侵染初期CLas在柑橘毛状根中的增长规律，优化发根农杆菌（Agrobacterium rhizogenes）介导的黄龙病抗性快速评价体系并应用于抗菌肽的抗性评价。【方法】基于植物表达载体pGNGM1300，通过发根农杆菌K599介导的毛状根转化体系转化不同柑橘品种，筛选毛状根诱导快、转化率高的...

文献信息：Yao, Lixiao, Guo, Xingru, Su, Juan, Zhang, Qingwen, Lian, Mengyao, Xue, Hao, Li, Qiang, He, Yongrui, Zou, Xiuping, Song, Zhen and Chen, Shanchun等.  请登录

文献信息：Fu, Jia, Zhang, Chenxi, Yu, Qiyuan, Xian, Baohang, Lin, Duo, Zhang, Miao, Zhong, Xin, Liu, Yiqi, Li, Man, He, Houzheng, Yang, Wanming, Chen, Shanchun,等.  请登录

文献信息：Wang, Chunqing, Ma, Zhimin, Zhou, Jinhuan, Li, Jiaxin, Cao, Peng, Song, Chenhu, Li, Chuxin, Wang, Xinliang, Bin, Yu, Zhou, Changyong and Song, Zhen等.  请登录

文献信息： Long, Qin, Zhang, Lehuan, Zhu, Tianxiang, Zhao, Shuyang, Zou, Changyu, Xu, Lanzhen, He, Yongrui, Chen, Shanchun and Zou, Xiuping等.  请登录

文献信息：Yu, Qiyuan, He, Houzheng, Xian, Baohang, Zhang, Chenxi, Zhong, Xin, Liu, Yiqi, Zhang, Miao, Li, Man, He, Yongrui, Chen, Shanchun, Li, Qiang and Hö等.  请登录

文献信息：Xian, Baohang, Fu, Jia, Su, Liyan, Yu, Qiyuan, Song, Qingwei, Zhang, Chenxi, Yang, Wanming, Lin, Duo, Zhang, Miao, Chen, Shanchun, He, Yongrui and Li,等.  请登录

文献信息：Gong, J. L., Chen, Y. S., Xu, Y. N., Gu, M. F., Ma, H. J., Hu, X. L., Li, X. L., Jiao, C. and Sun, X. P. Year（年份）: 2024等.  请登录

简介： Due to the protracted transgenic timeline and low efficiency in stable genetic transformation of woody plants, there has been limited exploration of real-time organelle imaging within stable transgenic woody plant cells. Here, we established an efficient in vivo genetic transformation system for woody plants using an Agrobacterium rhizogenes-mediated approach. This system was successfully validat...

文献信息：Zhang, Shushe, Wang, Xuefeng, Zhao, Tingchang and Zhou, Changyong等.  请登录

简介：Citrus huanglongbing (HLB) has been causing enormous damage to the global citrus industry. As the main causal agent, 'Candidatus Liberibacter asiaticus' (CLas) delivers a set of effectors to modulate host responses, while the modes of action adopted remain largely unclear. Here, we demonstrated that CLIBASIA_00185 (CLas0185) could attenuate reactive oxygen species (ROS)-mediated cell death in Nico...

文献信息：Gong, J. L., Chen, Y. S., Xu, Y. N., Gu, M. F., Ma, H. J., Hu, X. L., Li, X. L., Jiao, C. and Sun, X. P.等.  请登录

简介： Due to the protracted transgenic timeline and low efficiency in stable genetic transformation of woody plants, there has been limited exploration of real-time organelle imaging within stable transgenic woody plant cells. Here, we established an efficient in vivo genetic transformation system for woody plants using an Agrobacterium rhizogenes-mediated approach. This system was successfully validat...

文献信息：Zhang, Shushe, Wang, Xuefeng, Zhao, Tingchang and Zhou, Changyong等.  请登录

简介：Citrus huanglongbing (HLB) has been causing enormous damage to the global citrus industry. As the main causal agent, 'Candidatus Liberibacter asiaticus' (CLas) delivers a set of effectors to modulate host responses, while the modes of action adopted remain largely unclear. Here, we demonstrated that CLIBASIA_00185 (CLas0185) could attenuate reactive oxygen species (ROS)-mediated cell death in Nico...

文献信息：Su, Hang, Wang, Yuanchun, Xu, Jin, Omar, Ahmad A., Grosser, Jude W. and Wang, Nian等.  请登录

简介： Introduction: Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is a devastating disease worldwide. Previously, we successfully generated canker-resistant Citrus sinensis cv. Hamlin lines in the T0 generation. This was achieved through the transformation of embryogenic protoplasts using the ribonucleoprotein (RNP) containing Cas12a and one crRNA to edit the canker susceptibility gene...

文献信息：Sarkar, Poulami, Vazquez, Jorge Santiago, Zhou, Mingxi, Levy, Amit, Mou, Zhonglin and Orbovic, Vladimir等.  请登录

简介：Several expression systems have been developed in clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) framework allowing for gene editing of disease-associated genes across diverse citrus varieties. In this study, we present a new approach employing a multi-intron containing Cas9 gene plus multiple gRNAs separated with tRNA sequences to target the ...

文献信息： Nunez-Munoz, Leandro Alberto, Sanchez-Garcia, Martin Eduardo, Calderon-Perez, Berenice, De la Torre-Almaraz, Rodolfo, Ruiz-Medrano, Roberto and Xocon等.  请登录

简介：Huanglongbing, also known as citrus greening, is currently the most devastating citrus disease with limited success in prevention and mitigation. A promising strategy for Huanglongbing control is the use of antimicrobials fused to a carrier protein (phloem protein of 16 kDa or PP16) that targets vascular tIssues. This study investigated the effects of genetically modified citrus trees expressing C...

文献信息：Mahmoud, Lamiaa M. and Dutt, Manjul等.  请登录

简介： Key messageA lipofectamine-mediated transfection protocol for DNA-free genome editing of citrus protoplast cells using a Cas9/gRNA ribonucleoprotein (RNP) complex resulted in the production of transgene free genome edited citrus.

文献信息：Liu, Hao, Deng, Bin, Chen, Feng-Hao, Suo, Jia-Qi, Ouyang, Ge-Cheng, Lu, Hui-Lin, Chen, Da-Song and Meng, Xiang等.  请登录

简介：BACKGROUND: Citrus huanglongbing (HLB) is a devastating disease caused by Candidatus Liberibacter asiaticus (CLas) that affects the citrus industry. In nature, CLas relies primarily on Diaphorina citri Kuwayama as its vector for dissemination. After D. citri ingests CLas-infected citrus, the pathogen infiltrates the insect's body, where it thrives, reproduces, and exerts regulatory control over th...

文献信息：Li, Q., Xian, B., Yu, Q., Jia, R., Zhang, C., Zhong, X., Zhang, M., Fu, Y., Liu, Y., He, H., Li, M., Chen, S. and He, Y.等.  请登录

简介：Citrus bacterial canker (CBC) is a serious bacterial disease caused by Xanthomonas citri subsp. citri (Xcc) that adversely impacts the global citrus industry. In a previous study, we demonstrated that overexpression of an Xcc-inducible apetala 2/ethylene response factor encoded by Citrus sinensis, CsAP2-09, enhances CBC resistance. The mechanism responsible for this effect, however, is not known. ...

文献信息：Kishk, Abdelaziz, Stelinski, Lukasz L., Gowda, Siddarame and Killiny, Nabil等.  请登录

简介：BACKGROUND: Asian citrus psyllid, Diaphorina citri, is a hemipteran that vectors the causal pathogen of citrus greening disease, or huanglongbing (HLB). HLB is a tree killing disease that has severely limited citrus production globally. Unfortunately, there is no cure for this disease, and mitigation depends on multiple insecticide applications to reduce vector populations. Silencing of cytochrome...

文献信息：Jia, Hongge, Omar, Ahmad A., Xu, Jin, Dalmendray, Javier, Wang, Yuanchun, Feng, Yu, Wang, Wenting, Hu, Zhuyuan, Grosser, Jude W. and Wang, Nian等.  请登录

简介： Citrus canker disease affects citrus production. This disease is caused by Xanthomonas citri subsp. citri (Xcc). Previous studies confirmed that during Xcc infection, PthA4, a transcriptional activator like effector (TALE), is translocated from the pathogen to host plant cells. PthA4 binds to the effector binding elements (EBEs) in the promoter region of canker susceptibility gene LOB1 (EBEPthA4-...

文献信息：Huang, X., Su, L., Xian, B., Yu, Q., Zhang, M., Fan, J., Zhang, C., Liu, Y., He, H., Zhong, X., Li, M., Chen, S., He, Y. and Li, Q.等.  请登录

简介：Citrus bacterial canker (CBC) is a harmful bacterial disease caused by Xanthomonas citri subsp. citri (Xcc), negatively impacting citrus production worldwide. The basic helix-loop-helix (bHLH) transcription factor family plays crucial roles in plant development and stress responses. This study aimed to identify and annotate bHLH proteins encoded in the Citrus sinensis genome and explore their invo...